2: dicyclohexylcarbodiimide; 1-hydroxybenztriazole 3: hydrogen / Pd black View Scheme

whole cells of Rhodoccocus erythropolis NCIMB 11540 in phosphate buffer T=37°C; pH=8; 48 h; Enzymatic reaction;

1: NH4Cl / methanol; aq. NH3 / 5 h / 20 °C 2: 23.0 g / toluene; aq. NaOH / 1 h 3: 89 percent / cc. HCl / acetone / 1 h / 20 °C 4: MnSO4 / whole cells of Pseudomonas putida ATCC 12633 / H2O / 90 h / 37 °C / pH 8.3 / Enzymatic reaction View Scheme

1: 23.0 g / toluene; aq. NaOH / 1 h 2: 89 percent / cc. HCl / acetone / 1 h / 20 °C 3: MnSO4 / whole cells of Pseudomonas putida ATCC 12633 / H2O / 90 h / 37 °C / pH 8.3 / Enzymatic reaction View Scheme

1: 10percent aq. HCl / tetrahydrofuran / 1 h / Ambient temperature 2: NaIO4 / H2O; tetrahydrofuran / 48 h / Ambient temperature; pH 3 View Scheme

Yield given;