1: water; Candida antarctica lipase B SG202 mutant / aq. phosphate buffer; acetonitrile / 1 h / 30 °C / pH 7.5 / |Resolution of racemate; |Enzymatic reaction 2: Candida antarctica lipase B RG401 mutant; water / aq. phosphate buffer / 25 °C / pH 7.5 / |Enzymatic reaction View Scheme

1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 2: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 4: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 5: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 6: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 7: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 8: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C View Scheme

1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 2: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 4: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 5: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 6: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 7: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 8: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 9: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 10: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 11: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 12: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 13: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 14: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 15: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 16: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 17: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 18: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C View Scheme

1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 2: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 4: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 5: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 6: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 7: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 8: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 9: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 10: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 11: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 12: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 13: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 14: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 15: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 16: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 17: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 18: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 19: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 20: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 21: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 22: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 23: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 24: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C View Scheme

1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 2: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 4: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 5: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 6: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 7: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 8: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 9: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 10: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 11: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 12: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 13: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 14: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 15: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 16: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 17: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 18: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 19: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 20: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 21: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 22: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C View Scheme

1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 2: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 4: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 5: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 6: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 7: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 8: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 9: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 10: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 11: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 12: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 13: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 14: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 15: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 16: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 17: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 18: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 19: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 20: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C View Scheme