4-硝基苯基-β-D-纤维三糖苷合成路线 （共 21 条）
反应条件
Thermus nonproteolyticus HG102 E338A mutant; glycine; sodium hydroxide in aq. buffer T=45°C; pH=8.8; 48 h; Enzymatic reaction; Overall yield = 97 percent;
参考文献
European Journal of Organic Chemistry, , # 12 p. 2414 - 2419
反应条件
Thermus nonproteolyticus HG102 E338A mutant; glycine; sodium hydroxide in aq. buffer T=45°C; pH=8.8; 48 h; Enzymatic reaction; pH-valueConcentrationTemperature; Overall yield = 96 percent;
参考文献
European Journal of Organic Chemistry, , # 12 p. 2414 - 2419
反应条件
AbgGlu358Ala; ammonium bicarbonate buffer 8 h; Ambient temperature;
参考文献
Journal of the American Chemical Society, , vol. 120, # 22 p. 5583 - 5584
反应条件
1: 38 percent / AbgGlu358Ala, ammonium bicarbonate buffer / 8 h / Ambient temperature 2: 8 percent / AbgGlu358Ala, ammonium bicarbonate buffer / 8 h / Ambient temperature View Scheme
参考文献
Journal of the American Chemical Society, , vol. 120, # 22 p. 5583 - 5584
反应条件
1: methanol; sodium methylate / 20 °C 2: sodium hydroxide; Thermus nonproteolyticus HG102 E338A mutant; glycine / aq. buffer / 48 h / 45 °C / pH 8.8 / |Enzymatic reaction View Scheme
参考文献
European Journal of Organic Chemistry, , # 12 p. 2414 - 2419
反应条件
1: pyridine; hydrogen fluoride / -20 - 20 °C 2: methanol; sodium methylate / 20 °C 3: sodium hydroxide; Thermus nonproteolyticus HG102 E338A mutant; glycine / aq. buffer / 48 h / 45 °C / pH 8.8 / |Enzymatic reaction View Scheme
参考文献
European Journal of Organic Chemistry, , # 12 p. 2414 - 2419
反应条件
1: methanol; sodium methylate / 20 °C / pH 10 - 11 2: sodium hydroxide; Thermus nonproteolyticus HG102 E338A mutant; glycine / aq. buffer / 48 h / 45 °C / pH 8.8 / |Enzymatic reaction View Scheme
参考文献
European Journal of Organic Chemistry, , # 12 p. 2414 - 2419
反应条件
1: boron trifluoride diethyl etherate / dichloromethane / 0 - 20 °C / |Inert atmosphere 2: methanol; sodium methylate / 20 °C / pH 10 - 11 3: sodium hydroxide; Thermus nonproteolyticus HG102 E338A mutant; glycine / aq. buffer / 48 h / 45 °C / pH 8.8 / |Enzymatic reaction View Scheme
参考文献
European Journal of Organic Chemistry, , # 12 p. 2414 - 2419
反应条件
1: boron trifluoride diethyl etherate / dichloromethane / 0 - 20 °C / |Inert atmosphere 2: methanol; sodium methylate / 20 °C / pH 10 - 11 3: sodium hydroxide; Thermus nonproteolyticus HG102 E338A mutant; glycine / aq. buffer / 48 h / 45 °C / pH 8.8 / |Enzymatic reaction View Scheme
参考文献
European Journal of Organic Chemistry, , # 12 p. 2414 - 2419
反应条件
1: methanol; sodium methylate / 20 °C / pH 10 - 11 2: sodium hydroxide; Thermus nonproteolyticus HG102 E338A mutant; glycine / aq. buffer / 48 h / 45 °C / pH 8.8 / |Enzymatic reaction View Scheme
参考文献
European Journal of Organic Chemistry, , # 12 p. 2414 - 2419
