1: 32 mg / 1.) hesperidinase/ citrate-phosphate; 2.) n-BuOH / dimethylsulfoxide / 1.) pH=4.6, 74 deg C, 20 min; 2.) reflux, 20 min 2: 24 mg / 1) hesperidinase, 0.05 M McIlvaine buffer, 4percent β-D-glucose; 2.) n-BuOH / dimethylsulfoxide / 1.) 40 deg C, 5 min; 2.) reflux, 20 min View Scheme

sulfuric acid T=90°C; 0.5 h;

hesperidinase; phosphocitrate; butan-1-ol in dimethyl sulfoxide 1.) pH=4.6, 74 deg C, 20 min; 2.) reflux, 20 min;