Dithiothreitol; magnesium chloride T=30°C; pH=7; 48 h; aq. bufferEnzymatic reaction;

1: sodium methylate / methanol / 3 h / 20 °C 2: Dithiothreitol; magnesium chloride / 48 h / 30 °C / pH 7 / aq. buffer; Enzymatic reaction View Scheme

1: phosphoric acid / 2 h / 60 °C / Vacuum 2: sodium methylate / methanol / 3 h / 20 °C 3: Dithiothreitol; magnesium chloride / 48 h / 30 °C / pH 7 / aq. buffer; Enzymatic reaction View Scheme

in tetrahydrofuran; water 7 h; Ambient temperature; Yield given;