potassium hydroxide; Aspergillus acylase I pH 7.5-8.0;

phenylalanine ammonia-lyase in various solvent(s) T=30°C; pH=8.8; 96 h;

Taxus phenylalanine aminomutase enzyme in water; glycerol T=31°C; pH=8; 20 h; aq. phosphate bufferEnzymatic reaction;

1.1: NaH / dimethylformamide; various solvent(s) / 0.5 h / 20 °C 1.2: 80 percent / dimethylformamide; various solvent(s) / 7 h / 20 - 60 °C 2.1: NaOH / methanol / 20 h / 20 °C 3.1: toluene / 2 h / Heating 4.1: 75 percent / aq. LiOH / 2.5 h / 60 °C 5.1: 44 percent / phenylalanine ammonia-lyase / various solvent(s) / 96 h / 30 °C / pH 8.8 View Scheme

1.1: SOCl2; benzotriazole / CH2Cl2 2.1: NaH / dimethylformamide; various solvent(s) / 0.5 h / 20 °C 2.2: 80 percent / dimethylformamide; various solvent(s) / 7 h / 20 - 60 °C 3.1: NaOH / methanol / 20 h / 20 °C 4.1: toluene / 2 h / Heating 5.1: 75 percent / aq. LiOH / 2.5 h / 60 °C 6.1: 44 percent / phenylalanine ammonia-lyase / various solvent(s) / 96 h / 30 °C / pH 8.8 View Scheme

1: toluene / 2 h / Heating 2: 75 percent / aq. LiOH / 2.5 h / 60 °C 3: 44 percent / phenylalanine ammonia-lyase / various solvent(s) / 96 h / 30 °C / pH 8.8 View Scheme

1: NaOH / methanol / 20 h / 20 °C 2: toluene / 2 h / Heating 3: 75 percent / aq. LiOH / 2.5 h / 60 °C 4: 44 percent / phenylalanine ammonia-lyase / various solvent(s) / 96 h / 30 °C / pH 8.8 View Scheme

1: 75 percent / aq. LiOH / 2.5 h / 60 °C 2: 44 percent / phenylalanine ammonia-lyase / various solvent(s) / 96 h / 30 °C / pH 8.8 View Scheme

phenylalanine ammonia-lyase in various solvent(s) T=30°C; pH=8.8; Enzyme kinetics;