tetrafluoroboric acid diethyl ether complex; sodium sulfate 15 h; Ambient temperature;

sodium hydroxide T=20°C; 1.5 h; Enzymatic reaction;

anschliessendes Hydrieren;

sodium hydroxide; sodium hydrogencarbonate

ethanol; hydroxylamine

water; hydrazine hydrate

1: NEt3 / dimethylformamide; H2O / 4 h / Ambient temperature 2: 57 percent / BH3 / tetrahydrofuran / 13 h / -15 °C 3: 92 percent / NEt3, 4-(dimethylamino)pyridine / CH2Cl2 / 72 h / Ambient temperature 4: 77 percent / NaH / hexamethylphosphoric acid triamide / 0 deg C, 1 h; room temperature, 16 h 6: 75 percent / toluene / 40 h / 130 °C 7: tetrabutylammonium fluoride / tetrahydrofuran / 1 h / Ambient temperature 8: Jones' reagent / acetone / 0.33 h / 0 °C View Scheme

1: MgO / unter Kuehlung 2: hydrazine hydrate / 100 °C 3: NaNO2 / ueber mehrere Stufen 4: liquid ammonia; sodium View Scheme

recombinant Pseudomonas nitroreducens IFO12694 γ-glutamyltranspeptidase T=30°C; pH=10.5; 0.0333333 h; aq. bufferEnzymatic reaction;

water T=40°C; pH 7.6-10.4, μ=0.5 (KCl); Rate constant;