1: With trifluoroacetic acid in dichloromethane T=0 - 23°C; 2 h; 2: With sodium hydrogencarbonate in water pH=~ 8;

4-(N,N-dimethlyamino)pyridine; 1-ethyl-(3-(3-dimethylamino)propyl)-carbodiimide hydrochloride in N,N-dimethyl-formamide

sodium hydrogencarbonate in ethanol; water pH=8; 1.5 h;

trifluoroacetic acid in tetrahydrofuran T=20°C; 16 h;

1.1: (benzotriazo-1-yloxy)tris(dimethylamino)phosphonium hexafluorophosphate; pyridine / 48 h / 23 °C 2.1: trifluoroacetic acid / dichloromethane / 2 h / 0 - 23 °C 2.2: pH ~ 8 View Scheme

1.1: sodium hydride / N,N-dimethyl-formamide / 0.5 h / 0 °C / Inert atmosphere 1.2: 5 h / 23 °C 2.1: hydrazine hydrate; anhydrous iron chloride / methanol / 90 °C 3.1: (benzotriazo-1-yloxy)tris(dimethylamino)phosphonium hexafluorophosphate; pyridine / 48 h / 23 °C 4.1: trifluoroacetic acid / dichloromethane / 2 h / 0 - 23 °C 4.2: pH ~ 8 View Scheme

1.1: hydrazine hydrate; anhydrous iron chloride / methanol / 90 °C 2.1: (benzotriazo-1-yloxy)tris(dimethylamino)phosphonium hexafluorophosphate; pyridine / 48 h / 23 °C 3.1: trifluoroacetic acid / dichloromethane / 2 h / 0 - 23 °C 3.2: pH ~ 8 View Scheme

1.1: tetrahydrofuran / 18 h / 20 °C / Inert atmosphere 2.1: (benzotriazo-1-yloxy)tris(dimethylamino)phosphonium hexafluorophosphate; pyridine / 48 h / 23 °C 3.1: trifluoroacetic acid / dichloromethane / 2 h / 0 - 23 °C 3.2: pH ~ 8 View Scheme

Escherichia coli β-glucuronidase in phosphate buffer T=37°C; pH=7; Enzyme kinetics;

Escherichia coli β-glucuronidase in phosphate buffer T=37°C; pH=7; Enzyme kinetics;