Multi-step reaction with 5 steps 1: 74.3 percent / N,N'-dicyclohexylcarbodiimide / ethyl acetate / 24 h / Ambient temperature2: 88.3 percent / mesentericopeptidase / 6 h / 25 °C / pH = 8.03: phosphodiesterase I / 6 h / 37 °C / pH = 8.84: 86.3 percent / 2 N sodium hydroxide / 12 h / 5 °C5: α-chymotrypsin / 6 h / 25 °C / pH = 7.8  

Multi-step reaction with 2 steps 1: 86.3 percent / 2 N sodium hydroxide / 12 h / 5 °C2: α-chymotrypsin / 6 h / 25 °C / pH = 7.8  

Multi-step reaction with 3 steps 1: phosphodiesterase I / 6 h / 37 °C / pH = 8.82: 86.3 percent / 2 N sodium hydroxide / 12 h / 5 °C3: α-chymotrypsin / 6 h / 25 °C / pH = 7.8  

Multi-step reaction with 4 steps 1: 88.3 percent / mesentericopeptidase / 6 h / 25 °C / pH = 8.02: phosphodiesterase I / 6 h / 37 °C / pH = 8.83: 86.3 percent / 2 N sodium hydroxide / 12 h / 5 °C4: α-chymotrypsin / 6 h / 25 °C / pH = 7.8