N,N-bis-2-(hydroxyethyl)-2-amino ethane sulfonic acid; esterase from Streptomyces diastatochromogenes in water T=25°C; pH=7.20; Enzyme kinetics;

N,N-bis-2-(hydroxyethyl)-2-amino ethane sulfonic acid; esterase from Streptomyces diastatochromogenes in water T=25°C; pH=7.20; Enzyme kinetics;

<b>With</b>&nbsp; <a href="#" onclick="return RXDsubstances(event, this, '110354', constants.ctx, '0')">4-(N,N-dimethlyamino)pyridine</a>;&nbsp;<a href="#" onclick="return RXDsubstances(event, this, '605283', constants.ctx, '0')">triethylamine</a>;&nbsp;<a href="#" onclick="return RXDsubstances(event, this, '610662', constants.ctx, '0')">dicyclohexyl-carbodiimide</a>&nbsp;<b>in</b>&nbsp; <a href="#" onclick="return RXDsubstances(event, this, '1730800', constants.ctx, '0')">dichloromethane</a> <br />T=20&deg;C;&nbsp;24&nbsp;h;&nbsp;<br /> <br /> &nbsp;

<b>Multi-step reaction with 3 steps<br /> </b>1: 91 percent / DMAP, Na₂CO₃ / ethyl acetate<br />2: H₂O, Rhizopus oryzae lipase, BES buffer (pH 7.2), Triton X-100 / 16.5 h / 25 &deg;C / different time; various hydrolases<br />3: DMAP, Na₂CO₃ / ethyl acetate<br /> <br /> &nbsp;

<b>Multi-step reaction with 2 steps<br /> </b>1: H₂O, Rhizopus oryzae lipase, BES buffer (pH 7.2), Triton X-100 / 16.5 h / 25 &deg;C / different time; various hydrolases<br />2: DMAP, Na₂CO₃ / ethyl acetate<br /> <br /> &nbsp;

<b>With</b>&nbsp; <a href="#" onclick="return RXDsubstances(event, this, '103233', constants.ctx, '0')">pyridine</a> <br />1&nbsp;h;&nbsp;&nbsp;Ambient temperature;&nbsp;<br /> &nbsp;

<b>With</b>&nbsp; <a href="#" onclick="return RXDsubstances(event, this, '14235273', constants.ctx, '0')">novozyme 435</a>&nbsp;<b>in</b>&nbsp; <a href="#" onclick="return RXDsubstances(event, this, '1730733', constants.ctx, '0')">hexane</a> <br />T=20&deg;C;&nbsp;0.75&nbsp;h;&nbsp;&nbsp;Resolution of racemateEnzymatic reaction;&nbsp;&nbsp;Solvent;&nbsp;&nbsp;Overall yield = 39 percent; enantioselective reaction;&nbsp;<br /> &nbsp;