Rhodococcus sp. AJ270 in phosphate buffer T=30°C; pH=7.62; 3 h;

1: NH4Cl 2: 50 percent / Rhodococcus sp. AJ270 / aq. phosphate buffer / 3 h / 30 °C / pH 7.62 View Scheme

1.1: ammonium chloride 1.2: 24 h / 20 °C 2.1: hydrogenchloride / water / 3 h / 20 °C 3.1: PLP / aq. buffer / 36 h / 40 °C / pH 8.0 / |Enzymatic reaction View Scheme

potassium hydroxide; ammonia; (+)-(1S,2R)-N-hexadecyl-N-methylephedrinium bromide; lithium chloride in ethanol 5 deg C, 4 h, RT, overnight; asymmetric induction; Product distribution;