1: HBr / acetic acid 2: 87 percent / EDC*HCl; HOBT; Et3N / dimethylformamide / 20 °C 3: 4.2 percent Chromat. / Et3N; α-chymotrypsin immobilised on Celite / acetonitrile; various solvent(s) / 1 h / 30 °C / pH 7.8 View Scheme

Tris buffer; TEA; Celite; α-chymotrypsin in acetonitrile T=30°C; 1 h;

phosphate buffer; α-chymotrypsin in dimethyl sulfoxide T=30°C; pH=8.0; 0.5 h;

1: 17 percent / phosphate buffer; α-chymotrypsin / dimethylsulfoxide / 0.5 h / 30 °C / pH 8.0 2: BF3*OEt2 3: 80.8 percent / phosphate buffer; α-chymotrypsin / dimethylsulfoxide / 0.5 h / 30 °C / pH 8.0 View Scheme

1: 17 percent / phosphate buffer; α-chymotrypsin / dimethylsulfoxide / 0.5 h / 30 °C / pH 8.0 2: BF3*OEt2 View Scheme

<b>With</b>&nbsp; <a href="#" onclick="return RXDsubstances(event, this, '110354', constants.ctx, '0')">4-(N,N-dimethlyamino)pyridine</a>;&nbsp;<a href="#" onclick="return RXDsubstances(event, this, '5764110', constants.ctx, '0')">1-ethyl-(3-(3-dimethylamino)propyl)-carbodiimide hydrochloride</a>&nbsp;<b>in</b>&nbsp; <a href="#" onclick="return RXDsubstances(event, this, '1730800', constants.ctx, '0')">dichloromethane</a> <br />T=0 - 20&deg;C;&nbsp;<br /> &nbsp;

<b>Multi-step reaction with 2 steps<br /> </b>1: 96 percent / DMAP; EDC*HCl / CH₂Cl₂ / 0 - 20 &deg;C<br /> <br /> &nbsp;

<b>Multi-step reaction with 2 steps<br /> </b>1: 96 percent / DMAP; EDC*HCl / CH₂Cl₂ / 0 - 20 &deg;C<br /> <br /> &nbsp;