Enterobacter gergoviae CECT 875 in agar in phosphate buffer T=60°C; pH=7.0; 21 h; Enzymatic reaction;

1: 90 percent / H2, concd. NH4OH / 10percent Pd/C / methanol / 1.5 h / 775.7 Torr / Ambient temperature 2: 27 percent / tetra-n-butylammonium fluoride (TBAF) / tetrahydrofuran / 1 h / 5 - 10 °C View Scheme

ammonia 1.) acetonitrile, irradiation, 36 h, 2.) methanol, room temperature, 24 h; Yield given. Multistep reaction. Yields of byproduct given;

calf intestinal adenosine deaminase Deamination; Enzyme kinetics;

hydrogenchloride T=22°C; rate of hydrolysis relative to dideoxyadenosine;