Stage #1: With  Fe(III)PPIX(OH); recombinant human cyclooxygenase-2; tris(hydroxymethyl)methylamine hydrochloride; Aspirin; phenol T=20°C; pH=8; 0.0833333 h;  aq. bufferEnzymatic reaction; Stage #2: With  acetic acid in  methanol pH=4;  aq. buffer;