1: 65 percent / (-)-B-chlorodiisopinocampheylborane / tetrahydrofuran / 1 h / -4 °C 2: 70 percent / DIAD; PPh3 / tetrahydrofuran / 0 - 20 °C 3: 94 percent / NH2NH2*H2O / ethanol / 1.6 h / Heating View Scheme

[14C]-2-Ketoglutaric acid; Polaromonas sp. JS666 (S)-selective ω-transaminase; tris(hydroxymethyl)methylamine hydrochloride in water T=37°C; pH=7.5; Enzymatic reaction; enantioselective reaction;

1: 70 percent / DIAD; PPh3 / tetrahydrofuran / 0 - 20 °C 2: 94 percent / NH2NH2*H2O / ethanol / 1.6 h / Heating View Scheme

Yield given. Yields of byproduct given. Title compound not separated from byproducts;