pyridoxal 5'-phosphate T=30°C; 4 h; serine hydroxymethyltransferase (from rabbit liver cytosol, E. coli, E.coli mutant H228N), phosphate buffer (pH 7.50); stereochemistry of the decarboxylation of 2-amino-2-methylmalonic acid by serine hydroxymethyltramsferase; Product distribution;