Thermus nonproteolyticus HG102 E338A mutant; glycine; sodium hydroxide in aq. buffer T=45°C; pH=8.8; 48 h; Enzymatic reaction; Overall yield = 97 percent;

AbgGlu358Ala; ammonium bicarbonate buffer 8 h; Ambient temperature;

1: methanol; sodium methylate / 20 °C 2: sodium hydroxide; Thermus nonproteolyticus HG102 E338A mutant; glycine / aq. buffer / 48 h / 45 °C / pH 8.8 / |Enzymatic reaction View Scheme

1: pyridine; hydrogen fluoride / -20 - 20 °C 2: methanol; sodium methylate / 20 °C 3: sodium hydroxide; Thermus nonproteolyticus HG102 E338A mutant; glycine / aq. buffer / 48 h / 45 °C / pH 8.8 / |Enzymatic reaction View Scheme

1: methanol; sodium methylate / 20 °C / pH 10 - 11 2: sodium hydroxide; Thermus nonproteolyticus HG102 E338A mutant; glycine / aq. buffer / 48 h / 45 °C / pH 8.8 / |Enzymatic reaction View Scheme

1: hydrogen bromide; acetic acid / dichloromethane / 0 - 20 °C 2: tetra(n-butyl)ammonium hydrogensulfate; sodium hydroxide / water; dichloromethane / 20 °C 3: methanol; sodium methylate / 20 °C / pH 10 - 11 4: sodium hydroxide; Thermus nonproteolyticus HG102 E338A mutant; glycine / aq. buffer / 48 h / 45 °C / pH 8.8 / |Enzymatic reaction View Scheme

1: pyridine / 20 °C 2: hydrogen bromide; acetic acid / dichloromethane / 0 - 20 °C 3: tetra(n-butyl)ammonium hydrogensulfate; sodium hydroxide / water; dichloromethane / 20 °C 4: methanol; sodium methylate / 20 °C / pH 10 - 11 5: sodium hydroxide; Thermus nonproteolyticus HG102 E338A mutant; glycine / aq. buffer / 48 h / 45 °C / pH 8.8 / |Enzymatic reaction View Scheme

1: sodium hydroxide; Thermus nonproteolyticus HG102 E338A mutant; glycine / aq. buffer / 48 h / 45 °C / pH 8.8 / |Enzymatic reaction 2: sodium hydroxide; Thermus nonproteolyticus HG102 E338A mutant; glycine / aq. buffer / 48 h / 45 °C / pH 8.8 / |Enzymatic reaction View Scheme

1: methanol; sodium methylate / 20 °C / pH 10 - 11 2: sodium hydroxide; Thermus nonproteolyticus HG102 E338A mutant; glycine / aq. buffer / 48 h / 45 °C / pH 8.8 / |Enzymatic reaction 3: sodium hydroxide; Thermus nonproteolyticus HG102 E338A mutant; glycine / aq. buffer / 48 h / 45 °C / pH 8.8 / |Enzymatic reaction View Scheme

1: boron trifluoride diethyl etherate / dichloromethane / 0 - 20 °C / |Inert atmosphere 2: methanol; sodium methylate / 20 °C / pH 10 - 11 3: sodium hydroxide; Thermus nonproteolyticus HG102 E338A mutant; glycine / aq. buffer / 48 h / 45 °C / pH 8.8 / |Enzymatic reaction 4: sodium hydroxide; Thermus nonproteolyticus HG102 E338A mutant; glycine / aq. buffer / 48 h / 45 °C / pH 8.8 / |Enzymatic reaction View Scheme