1: sodium hydroxide / methanol / 20 °C 2: toluene-4-sulfonic acid / toluene / Reflux 3: sodium hydride / N,N-dimethyl-formamide / 5 h / -15 - 20 °C View Scheme

<b>With</b>&nbsp; Rhodotorula mucilaginosa DQ₈₃₂₁₉₈ <br />T=28&deg;C;&nbsp;pH=6;&nbsp;12&nbsp;h;&nbsp;&nbsp;Microbiological reactionphosphate-citric acid buffer;&nbsp;&nbsp;enantioselective reaction;&nbsp;<br /> &nbsp;

<b>Multi-step reaction with 4 steps<br /> </b>1: potassium carbonate / acetonitrile / 6 h / Reflux<br />2: methanol / 7 h / 20 &deg;C<br />3: pyridine; triethylamine / 20 &deg;C<br />4: Rhodotorula mucilaginosa DQ₈₃₂₁₉₈ / 12 h / 28 &deg;C / pH 6 / Microbiological reaction; phosphate-citric acid buffer<br /> <br /> &nbsp;

<b>Multi-step reaction with 3 steps<br /> </b>1: methanol / 7 h / 20 &deg;C<br />2: pyridine; triethylamine / 20 &deg;C<br />3: Rhodotorula mucilaginosa DQ₈₃₂₁₉₈ / 12 h / 28 &deg;C / pH 6 / Microbiological reaction; phosphate-citric acid buffer<br /> <br /> &nbsp;