pyridoxal 5'-phosphate; sodium 2-oxopropanoate in aq. phosphate buffer; dimethyl sulfoxide T=30°C; pH=7; 24 h; Resolution of racemateSchlenk techniqueInert atmosphereEnzymatic reaction; Reagent/catalyst;

1: water; acetone / 3.25 h / 20 °C / Reflux 2: sodium carbonate / water / pH 8 - 9 View Scheme

1: water; acetone / 3.25 h / 20 °C / Reflux 2: sodium carbonate / water / pH 8 - 9 3: sodium carbonate / ethanol; tert-butyl alcohol / 42 h / 50 - 90 °C 4: sodium hydroxide; water / methanol / 1.25 h / 20 °C / cooling with ice View Scheme

1: water; acetone / 3.25 h / 20 °C / Reflux 2: sodium carbonate / water / pH 8 - 9 3: sodium carbonate / ethanol; tert-butyl alcohol / 42 h / 50 - 90 °C 4: sodium hydroxide; water / methanol / 1.25 h / 20 °C / cooling with ice 5: sodium hydroxide; dihydrogen peroxide / water; dimethyl sulfoxide / 2.25 h / 20 - 25 °C View Scheme

in water; acetone T=25 - 65°C; Resolution of racemate;

in tetrahydrofuran; water T=20 - 65°C; Product distribution / selectivity;