in water 20 h; Ambient temperatureadenosine deaminase;

in water T=37 - 50°C; 48 h; thymidine phosphorylase, purine nucleoside phosphorylase, phosphate buffer, pH 7;

sodium methylate in methanol 1 h; Ambient temperature;

1: 23 percent / p-TsOH / 0.83 h / 160 °C / 25 Torr 2: 75 percent / NaN3 / ethanol; H2O / 1.) reflux, 1 h, 2.) room temperature, 20 h 3: 94 percent / H2 / Pd on carbon / ethanol; N,N-dimethyl-acetamide / 20 h / Ambient temperature 4: 79 percent / MeONa / methanol / 1 h / Ambient temperature View Scheme

1: 94 percent / H2 / Pd on carbon / ethanol; N,N-dimethyl-acetamide / 20 h / Ambient temperature 2: 79 percent / MeONa / methanol / 1 h / Ambient temperature View Scheme

1: 75 percent / NaN3 / ethanol; H2O / 1.) reflux, 1 h, 2.) room temperature, 20 h 2: 94 percent / H2 / Pd on carbon / ethanol; N,N-dimethyl-acetamide / 20 h / Ambient temperature 3: 79 percent / MeONa / methanol / 1 h / Ambient temperature View Scheme

1: 59 percent / mercuric cyanide / various solvent(s) / 24 h / Ambient temperature 2: 75 percent / NaN3 / ethanol; H2O / 1.) reflux, 1 h, 2.) room temperature, 20 h 3: 94 percent / H2 / Pd on carbon / ethanol; N,N-dimethyl-acetamide / 20 h / Ambient temperature 4: 79 percent / MeONa / methanol / 1 h / Ambient temperature View Scheme

E. coli BMT-4D Substitution;