1: 83.3 percent / 1N NaOH / ethanol / 16 h / 20 °C 2: 89.8 percent / xylene / 2 h / Heating 3: 95.8 percent / 24 h / 37 °C / α-chymotrypsin, 1N NaOH, 0.1M phosphate buffer, pH 7.5-8 View Scheme