1: With lithium hydroxide hydrate in tetrahydrofuran; water T=15 - 20°C; 4.25 h; Inert atmosphere; 2: With hydrogenchloride in tetrahydrofuran; water pH=2.5 - 3.5; 2 h; Inert atmosphere;

<b>Multi-step reaction with 5 steps<br /> </b>1.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 2.33 h / 65 - 67 &deg;C / Inert atmosphere<br />1.2: 3 h / 10 - 20 &deg;C / Inert atmosphere<br />2.1: di-μ-chloro-bis(η4-1,5-cyclooctadiene)diiridium / N,N-dimethyl-formamide / 7 h / 85 &deg;C / Inert atmosphere<br />3.1: CDX-017 enzyme; pyridoxal 5'-phosphate / water; dimethyl sulfoxide / 52 h / 45 &deg;C / pH 8.5 / Inert atmosphere; triethanolamine/isopropylamine buffer; Enzymatic reaction<br />3.2: 2 h / pH 6 / Inert atmosphere<br />4.1: sodium carbonate / isopropanol acetate; water / 0.08 h / 20 &deg;C / Inert atmosphere<br />4.2: 2 h / 20 &deg;C / Inert atmosphere<br />5.1: potassium carbonate / N,N-dimethyl-formamide / 2.08 h / Inert atmosphere<br /> <br /> &nbsp;

<b>Multi-step reaction with 5 steps<br /> </b>1.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 0.25 h / -25 - -20 &deg;C / Inert atmosphere<br />2.1: toluene / 1.17 h / Reflux; Inert atmosphere<br />3.1: CDX-017 enzyme; pyridoxal 5'-phosphate / water; dimethyl sulfoxide / 52 h / 45 &deg;C / pH 8.5 / Inert atmosphere; triethanolamine/isopropylamine buffer; Enzymatic reaction<br />3.2: 2 h / pH 6 / Inert atmosphere<br />4.1: sodium carbonate / isopropanol acetate; water / 0.08 h / 20 &deg;C / Inert atmosphere<br />4.2: 2 h / 20 &deg;C / Inert atmosphere<br />5.1: potassium carbonate / N,N-dimethyl-formamide / 2.08 h / Inert atmosphere<br /> <br /> &nbsp;

<b>Multi-step reaction with 3 steps<br /> </b>1.1: CDX-017 enzyme; pyridoxal 5'-phosphate / water; dimethyl sulfoxide / 52 h / 45 &deg;C / pH 8.5 / Inert atmosphere; triethanolamine/isopropylamine buffer; Enzymatic reaction<br />1.2: 2 h / pH 6 / Inert atmosphere<br />2.1: sodium carbonate / isopropanol acetate; water / 0.08 h / 20 &deg;C / Inert atmosphere<br />2.2: 2 h / 20 &deg;C / Inert atmosphere<br />3.1: potassium carbonate / N,N-dimethyl-formamide / 2.08 h / Inert atmosphere<br /> <br /> &nbsp;

<b>Multi-step reaction with 4 steps<br /> </b>1.1: di-μ-chloro-bis(η4-1,5-cyclooctadiene)diiridium / N,N-dimethyl-formamide / 7 h / 85 &deg;C / Inert atmosphere<br />2.1: CDX-017 enzyme; pyridoxal 5'-phosphate / water; dimethyl sulfoxide / 52 h / 45 &deg;C / pH 8.5 / Inert atmosphere; triethanolamine/isopropylamine buffer; Enzymatic reaction<br />2.2: 2 h / pH 6 / Inert atmosphere<br />3.1: sodium carbonate / isopropanol acetate; water / 0.08 h / 20 &deg;C / Inert atmosphere<br />3.2: 2 h / 20 &deg;C / Inert atmosphere<br />4.1: potassium carbonate / N,N-dimethyl-formamide / 2.08 h / Inert atmosphere<br /> <br /> &nbsp;

<b>Multi-step reaction with 2 steps<br /> </b>1.1: sodium carbonate / isopropanol acetate; water / 0.08 h / 20 &deg;C / Inert atmosphere<br />1.2: 2 h / 20 &deg;C / Inert atmosphere<br />2.1: potassium carbonate / N,N-dimethyl-formamide / 2.08 h / Inert atmosphere<br /> <br /> &nbsp;

<b>With</b>&nbsp; <a href="#" onclick="return RXDsubstances(event, this, '4267587', constants.ctx, '0')">potassium carbonate</a>&nbsp;<b>in</b>&nbsp; <a href="#" onclick="return RXDsubstances(event, this, '605365', constants.ctx, '0')">N,N-dimethyl-formamide</a> <br />2.08333&nbsp;h;&nbsp;&nbsp;Inert atmosphere;&nbsp;<br /> &nbsp;

<b>Multi-step reaction with 7 steps<br /> </b>1.1: toluene / 16 h / Reflux; Inert atmosphere<br />2.1: sulfuric acid / 2.5 h / 90 - 92 &deg;C / Inert atmosphere<br />2.2: 5 - 18 &deg;C / pH 7 / Inert atmosphere<br />3.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 2.33 h / 65 - 67 &deg;C / Inert atmosphere<br />3.2: 3 h / 10 - 20 &deg;C / Inert atmosphere<br />4.1: di-μ-chloro-bis(η4-1,5-cyclooctadiene)diiridium / N,N-dimethyl-formamide / 7 h / 85 &deg;C / Inert atmosphere<br />5.1: CDX-017 enzyme; pyridoxal 5'-phosphate / water; dimethyl sulfoxide / 52 h / 45 &deg;C / pH 8.5 / Inert atmosphere; triethanolamine/isopropylamine buffer; Enzymatic reaction<br />5.2: 2 h / pH 6 / Inert atmosphere<br />6.1: sodium carbonate / isopropanol acetate; water / 0.08 h / 20 &deg;C / Inert atmosphere<br />6.2: 2 h / 20 &deg;C / Inert atmosphere<br />7.1: potassium carbonate / N,N-dimethyl-formamide / 2.08 h / Inert atmosphere<br /> <br /> &nbsp;