esterase enzyme T=37°C; pH=7.4; Aqueous phosphate bufferEnzymatic reaction;

<b>Multi-step reaction with 2 steps<br /> </b>1: sodium L-ascorbate; CuSO_4‵5H₂O / N,N-dimethyl-formamide; water; <i>tert</i>-butyl alcohol / 0.17 h / 80 &deg;C / Microwave irradiation<br />2: an ion exchange resin / acetonitrile; water / 20 &deg;C<br /> <br /> &nbsp;

<b>Multi-step reaction with 4 steps<br /> </b>1: tetra(n-butyl)ammonium hydrogensulfate; sodium hydrogencarbonate / dichloromethane; water / 1 h / 20 &deg;C<br />2: caesium carbonate / N,N-dimethyl-formamide / 0.25 h / 50 &deg;C / Microwave irradiation<br />3: CuSO_4‵5H₂O; sodium L-ascorbate / water; N,N-dimethyl-formamide; <i>tert</i>-butyl alcohol / 0.17 h / 50 &deg;C / Microwave irradiation<br />4: ion exchange resin DIAION WK₁₁ / water; acetonitrile<br /> <br /> &nbsp;