hydrogenchloride in 1,4-dioxane 3 h; Ambient temperature;

1: acetonitrile 2: triethylamine / 18-crown-6 / CH2Cl2 3: 0.44 g / malonic acid, pyridine / ethanol / 2 h / Heating View Scheme

1: triethylamine / 18-crown-6 / CH2Cl2 2: 0.44 g / malonic acid, pyridine / ethanol / 2 h / Heating View Scheme

NaPi buffer; oxygen T=37°C; 0.166667 h; catalase and polyamine oxidase presence, pH 6.5, other polyamines and acetylpolyamines, kinetic constants Km (μM) and Vmax (μmol H2O2 formed μg protein-1 min-1);