methanol; ammonia; water; esterase SEQ ID NO:4 (encoded by SEQ ID NO:3) in toluene pH=7; Tris bufferEnzymatic reaction; Product distribution / selectivity;
1: With methanol; ammonia; water; hydrolase SEQ ID NO:4 (encoded by SEQ ID NO:3) in toluene T=50°C; pH=6.75; tris bufferEnzymatic reaction; 2: With hydrogenchloride in water pH=2.5; Product distribution / selectivity;
methanol; ammonia; water; esterase SEQ ID NO:4 (encoded by SEQ ID NO:3) in toluene pH=7; Tris bufferEnzymatic reaction; Product distribution / selectivity;
