1: acetic acid / water / 3 h / 20 °C 2: neat (no solvent) / 6 h / 20 °C 3: hydrogenchloride; bovine spleen phosphodiesterase II; succinic acid disodium salt / 20 h / 37 °C / pH 6.5 / |Enzymatic reaction View Scheme

1: acetic acid / water / 3 h / 20 °C 2: neat (no solvent) / 6 h / 20 °C 3: sodium chloride; magnesium chloride; Crotalus adamanteus snake venom phosphodiesterase I; tris(hydroxymethyl)methylamine hydrochloride / 48 h / 37 °C / pH 8.9 / |Enzymatic reaction View Scheme