1: With Uro'gen III synthase; potassium chloride; magnesium chloride in various solvent(s) T=37°C; pH=7.5 - 8.0; Enzymatic reaction; 2: With oxygen in various solvent(s) T=20°C; 1 h;

1.1: NaBH4 / H2O / 1 h / 20 °C 2.1: Uro'gen III synthase; MgCl2*6H2O; KCl / various solvent(s) / 37 °C / pH 7.5 - 8.0 / Enzymatic reaction 2.2: O2 / various solvent(s) / 1 h / 20 °C View Scheme

1.1: aq. KOH / methanol / 20 °C 2.1: NaBH4 / H2O / 1 h / 20 °C 3.1: Uro'gen III synthase; MgCl2*6H2O; KCl / various solvent(s) / 37 °C / pH 7.5 - 8.0 / Enzymatic reaction 3.2: O2 / various solvent(s) / 1 h / 20 °C View Scheme

succinic acid

succinic acid

1.1: sodium amalgam 2.1: recombinant oxygen-independent coproporphyrinogen III oxidase; [14C]-Ademetionine; Reduced nicotinamide-adenine dinucleotide; threo-1,4-dimercapto-2,3-butanediol / 0.5 h / 37 °C / pH 7 / Inert atmosphere; Bis-Tris buffer; Enzymatic reaction 2.2: 0.25 h / Bis-Tris buffer 3.1: sodium amalgam; potassium hydroxide / 0.08 h / 80 °C / Darkness 4.1: recombinant oxygen-independent coproporphyrinogen III oxidase; [14C]-Ademetionine; Reduced nicotinamide-adenine dinucleotide; threo-1,4-dimercapto-2,3-butanediol / 1 h / 37 °C / pH 7 / Inert atmosphere; Bis-Tris buffer; Enzymatic reaction 4.2: 0.25 h / Bis-Tris buffer View Scheme

1.1: sodium amalgam 2.1: recombinant oxygen-independent coproporphyrinogen III oxidase; [14C]-Ademetionine; Reduced nicotinamide-adenine dinucleotide; threo-1,4-dimercapto-2,3-butanediol / 0.5 h / 37 °C / pH 7 / Inert atmosphere; Bis-Tris buffer; Enzymatic reaction 2.2: 0.25 h / Bis-Tris buffer View Scheme

sodium amalgam