Alcalase-cross-linked-enzyme-aggregates in tert-butyl alcohol T=37°C; pH=7.5; 16 h; aq. phosphate bufferEnzymatic reaction; regioselective reaction;

sodium carbonate in 1,4-dioxane; water Ambient temperature;

1: Fmoc–Asp(OAll)–OH With N-ethyl-N,N-diisopropylamine in dichloromethane 3 h; 2-chlorotrityl chloride polystyrene resin; 2: With piperidine in 1-methyl-pyrrolidin-2-one 0.416667 h; 3: N-(9-fluorenylmethoxycarbonyl)glycine; N-(fluoren-9-ylmethoxycarbonyl)-L-Lys(Nε-Boc); FMOC-D-Phenylalanine; fmoc-L-Arg(Pbf)-OH Further stages;