反应条件
1.1: N-ethyl-N,N-diisopropylamine / dichloromethane / 0.5 h / 0 - 10 °C / Inert atmosphere 1.2: 1.5 h / 0 - 10 °C 2.1: tetrakis(triphenylphosphine) palladium(0) / N,N-dimethyl-formamide / 15 h / 70 °C / Inert atmosphere 3.1: Reduced nicotinamide-adenine dinucleotide; NADPH; Lycopersicon esculentum 12-oxophytodienoate reductase / dimethyl sulfoxide / 22 h / 30 °C / pH 7 / Enzymatic reaction; Aqueous phosphate buffer 4.1: potassium hydroxide; water / 6 h / 25 °C 5.1: hydrogen / Raney Nickel / water / 30 °C 6.1: acetic acid / ethanol / 35 °C / pH 7.5 View Scheme
