phosphate buffer; α-chymotrypsin in N,N-dimethyl-formamide T=25°C; steady-state 16O/18O KIE, pH and time dependence;

α-chymotrypsin in N,N-dimethyl-formamide 0.0583333 h; mass spectrometric product detection; Product distributionRate constant;

tris-buffer; hydroxide; copper in water; acetonitrile T=30°C; effect of added metal ion on rate of hydrolysis; pH=9; other catalysts and also without catalyst; Rate constantMechanism;