1: (i) SOCl2, (ii) /BRN= 2217949/, Et3N 2: H2, aq. NH3 / Raney-Ni View Scheme

nickel monoaluminide in methanol; water; acetone

dipotassium hydrogenphosphate; potassium dihydrogenphosphate; Acremonium sp. D9K; thiamine chloride hydrochloride; magnesium sulfate; glycerol; sodium chloride; 5-[(3AS,4S,6AR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoic acid in water T=30°C; at different pHs, effect of various carbon and nitrogen sources; Product distribution;