trifluoroacetic acid in dichloromethane T=20°C;

hydrogen bromide in acetic acid

endopeptidase in various solvent(s) T=25°C; 1 h; pH=8.0; apparent rate of hydrolysis;

phosphate-buffered saline pH=7.8; water T=37°C; rate of hydrolysis of various p-NA-substituted amino acids by human fibroblast aminopeptidases;

water in acetone Kinetics;

phosphate-buffered saline pH=7.8; water T=37°C; rate of hydrolysis of various p-NA-substituted amino acids by human fibroblast aminopeptidases;

1: 80.2 percent / triethylamine / dimethylformamide; dioxane / 4 °C 2: hydrogen bromide / acetic acid 3: 55.1 percent / triethylamine / dimethylformamide; tetrahydrofuran / 4 °C 4: hydrogen bromide / acetic acid 5: 78.9 percent / triethylamine / dimethylformamide / Ambient temperature 6: hydrogen bromide / acetic acid 7: 66.5 percent / pyridine, triethylamine / 4 °C View Scheme

MOPS buffer; calcium chloride; bovine trypsin in dimethyl sulfoxide T=25°C; 5 h;

4-morpholinepropanesulfonate buffer; methyltrypsin in water; dimethyl sulfoxide T=25°C; 4 h;

Streptomyces griseus trypsin in dimethyl sulfoxide T=25°C; pH=8.0; 24 h;