1: Tris buffer pH 8.2 / 168 h / Ambient temperature; L-leucine aminopeptidase 2: NH3 / methanol / 72 h / 0 °C 3: methanol / 1 h / Ambient temperature View Scheme

in methanol 1 h; Ambient temperature; Yield given. Yields of byproduct given. Title compound not separated from byproducts;