potassium hydroxide Ambient temperaturepenicillin acylase (EC 3.5.1.11), pH 7.5;

2: 6 N aq. HCl / 11 h / 50 °C View Scheme

<b>Multi-step reaction with 2 steps<br /> </b>1: ammonium acetate / ethanol / 6 h / |Reflux<br />2: triethylamine / acetone; water / 5.5 h / -5 - 20 &deg;C<br /> <br /> &nbsp;