hydrogenchloride; sodium nitrite 48 h; Ambient temperature;

1: H2O / ethanol / 60 °C 2: 79 percent / 0.25 M Tris-HCl buffer, MnCl2 / 24 h / 50 °C / D-hydantoinase-1 3: 65 percent / NaNO2, 3.5 N aq. HCl / 48 h / Ambient temperature View Scheme

1: 79 percent / 0.25 M Tris-HCl buffer, MnCl2 / 24 h / 50 °C / D-hydantoinase-1 2: 65 percent / NaNO2, 3.5 N aq. HCl / 48 h / Ambient temperature View Scheme

potassium hydroxide; ammonia; (+)-(1S,2R)-N-hexadecyl-N-methylephedrinium bromide; lithium chloride in ethanol 5 deg C, 4 h, RT, overnight; asymmetric induction; Product distribution;