1: sec-alkylsulfatase Pisa1 / 24 h / 30 °C / pH 8.2 / aq. buffer; Enzymatic reaction 2: toluene-4-sulfonic acid / 1,4-dioxane; tert-butyl methyl ether; water / 2 h / 40 °C View Scheme

sec-alkylsulfatase Pisa1 T=30°C; pH=8.2; 24 h; aq. bufferEnzymatic reaction; optical yield given as percent ee;