recombinant His-tagged Streptococcus mutans ATCC25175 L-amino acid α-ligase; water; tris(hydroxymethyl)methylamine hydrochloride; magnesium sulfate; adenosine 5'-triphosphoric acid T=37°C; pH=8; 16 h; aq. bufferEnzymatic reaction;

potassium hydroxide; [bis(acetoxy)iodo]benzene T=0 - 5°C; 1.5 h;