wildtype P450BM3; NADPH; catalase in phosphate buffer T=37°C; 0.5 h; Title compound not separated from byproducts;

potassium hydroxide in methanol

oxygen; NADPH; monooxygenase CYP102A1 mutant A74G,F87V,L188Q,R966D,W1046S in phosphate buffer; N-dodecane T=20°C; pH=6; 24 h; Enzyme kinetics; Further Variations:Reagents;

1.1: 64.6 percent / diethyl ether / 1 h 2.1: borane / tetrahydrofuran / 2 h / 0 °C 2.2: 78 percent / aq NaOH; 30 percent aq H2O2 / 0.5 h / 45 °C 3.1: tetra-n-butylammonium chlorde; 2.2.6.6-tetramethylpipridin-1-yloxy radical (TEMPO); N-chlorosuccinimide / CH2Cl2; various solvent(s) / 1.5 h / pH 8.6 4.1: NaClO2 / 2-methyl-propan-2-ol; various solvent(s); H2O / 20 °C View Scheme

1.1: borane / tetrahydrofuran / 2 h / 0 °C 1.2: 78 percent / aq NaOH; 30 percent aq H2O2 / 0.5 h / 45 °C 2.1: tetra-n-butylammonium chlorde; 2.2.6.6-tetramethylpipridin-1-yloxy radical (TEMPO); N-chlorosuccinimide / CH2Cl2; various solvent(s) / 1.5 h / pH 8.6 3.1: NaClO2 / 2-methyl-propan-2-ol; various solvent(s); H2O / 20 °C View Scheme

1: tetra-n-butylammonium chlorde; 2.2.6.6-tetramethylpipridin-1-yloxy radical (TEMPO); N-chlorosuccinimide / CH2Cl2; various solvent(s) / 1.5 h / pH 8.6 2: NaClO2 / 2-methyl-propan-2-ol; various solvent(s); H2O / 20 °C View Scheme

acetic acid; platinum T=50°C; Hydrogenation;

cytochrome P450 BM3 Enzymatic reaction;

glucose-6-phosphate dehydrogenase; glucose-6-phosphate; Sorangium cellulosum So ce56 cytochrome P109D1; Sorangium cellulosum So ce56 His-tagged ferredoxin reductase; NADP; magnesium chloride 0.5 h; aq. bufferEnzymatic reaction; regioselective reaction;

cytochrome P450BM3 Enzymatic reaction;