potassium phosphate buffer; Proteus mirabilis IFO 3849 in water T=30°C; 7 h;

1: 2-hydroxymuconic 6-semialdehyde dehydrogenase; NAD+ / various solvent(s) / 1 h / 24 °C / pH 7.5 2: 4-oxalocrotonate tautomerase / various solvent(s) / 24 °C / pH 7.0 3: MgSO4; 4-oxalocrotonate decarboxylase / various solvent(s) / 1 h / 24 °C / pH 8.0 View Scheme

hydroxylase MhpC T=20°C; pH 5.0;

NaH2PO4 buffer T=23°C; also enzymatic isomerization with 4-oxalocrotonate tautomerase; Rate constant;

1: 2 N HCl 2: hydrogen / Adams platinum catalyst / ethanol / 1 h View Scheme