1: Inert atmosphere 2: {RuCl2(1-methyl-4-(1-methylethyl)benzene)}2; potassium tert-butylate; 2-amino-2-methyl-propan-1-ol; isopropyl alcohol / 2.5 h / 50 °C / Inert atmosphere; Molecular sieve 3: hydrogenchloride / methanol / 20 °C View Scheme

[3H]-L-Alanine; alanine dehydrogenase; formate dehydrogenase from Candida boidinii; ω-transaminase from variant Arthrobacter citreus; PLP; ammonium formate; β-NADP+ in aq. phosphate buffer T=30°C; pH=7; 24 h; Enzymatic reaction; enantioselective reaction;

Yield given. Multistep reaction. Yields of byproduct given;