1: 1.) acyl azide formation, 2.) Curtius rearrangement 2: aq. HBr (48 percent) View Scheme

<b>Multi-step reaction with 2 steps<br /> </b>1: NaH / tetrahydrofuran / 0 - 25 &deg;C<br />2: α-chymotrypsin / dimethylsulfoxide; H₂O / 22 - 25 &deg;C / potassium phosphate ( pH 7.0); with pig liver esterase the reaction rate is higher but the e.e. is lower<br /> <br /> &nbsp;

<b>Multi-step reaction with 2 steps<br /> </b>1: NaH / tetrahydrofuran / 0 - 25 &deg;C<br />2: α-chymotrypsin / dimethylsulfoxide; H₂O / 22 - 25 &deg;C / potassium phosphate ( pH 7.0); with pig liver esterase the reaction rate is higher but the e.e. is lower<br /> <br /> &nbsp;