1: recombinant Arabidopsis thaliana UGT71B6; Tris-HCl buffer / H2O / 1 h / 30 °C / pH 7.0 2: β-glucosidase; sodium phosphate / H2O / 1 h / 37 °C / pH 5.0 View Scheme

Tris-HCl buffer; recombinant Arabidopsis thaliana UGT71B6 in water T=30°C; pH=7.0; 1 h;

Escherichia coli BL21 expressing UGT71B6; isopropyl 1-thio-β-D-galactopyranoside; sodium salt of phosphorous acid in water T=28°C; pH=7.4; 24 h;

sodium hydroxide; silver nitrate in methanol

1: 22 mg / Jones reagent / acetone / 0.05 h 2: toluene / 4 h / Heating 3: 2M KOH / methanol / 23 h / Ambient temperature View Scheme

1: toluene / 4 h / Heating 2: 2M KOH / methanol / 23 h / Ambient temperature View Scheme

1: MnO2 / CH2Cl2 / 3 h / Ambient temperature 2: TsOH / CH2Cl2 / 1.5 h / Ambient temperature 3: 1.) 1.5 M BuLi, 2.) 3.5 M natrium-dihydro-bis(2-methoxyethoxy)aluminate, 3.) H2O, pyridinium p-toluenesulfonate / 1.) THF/hexane, -15 deg C, 15 min, 2.) THF/hexane/toluene, -45 deg C, 1 h, 3.) THF, 2 h, room temperature 4: 21.9 g / MnO2 / CH2Cl2 / 2 h / Ambient temperature 5: NaClO2, NaH2PO4*2H2O / 2-methyl-propan-2-ol; various solvent(s) / 1 h / Ambient temperature View Scheme

1: TsOH / CH2Cl2 / 1.5 h / Ambient temperature 2: 1.) 1.5 M BuLi, 2.) 3.5 M natrium-dihydro-bis(2-methoxyethoxy)aluminate, 3.) H2O, pyridinium p-toluenesulfonate / 1.) THF/hexane, -15 deg C, 15 min, 2.) THF/hexane/toluene, -45 deg C, 1 h, 3.) THF, 2 h, room temperature 3: 21.9 g / MnO2 / CH2Cl2 / 2 h / Ambient temperature 4: NaClO2, NaH2PO4*2H2O / 2-methyl-propan-2-ol; various solvent(s) / 1 h / Ambient temperature View Scheme

1: 1.) 1.5 M BuLi, 2.) 3.5 M natrium-dihydro-bis(2-methoxyethoxy)aluminate, 3.) H2O, pyridinium p-toluenesulfonate / 1.) THF/hexane, -15 deg C, 15 min, 2.) THF/hexane/toluene, -45 deg C, 1 h, 3.) THF, 2 h, room temperature 2: 21.9 g / MnO2 / CH2Cl2 / 2 h / Ambient temperature 3: NaClO2, NaH2PO4*2H2O / 2-methyl-propan-2-ol; various solvent(s) / 1 h / Ambient temperature View Scheme

1: 21.9 g / MnO2 / CH2Cl2 / 2 h / Ambient temperature 2: NaClO2, NaH2PO4*2H2O / 2-methyl-propan-2-ol; various solvent(s) / 1 h / Ambient temperature View Scheme