1: 76 percent / toluene / Heating 2: β-alanine; glacial acetic acid / hexane / Heating 3: 3-ethyl-5-(2-hydroxyethyl)-4-methylthiazolium bromide; triethylamine / ethanol / Heating 4: pivalic acid / tetrahydrofuran; toluene; heptane / Heating 5: 35 percent / hydrogen; acetic acid / palladium on carbon / ethyl acetate 6: HCl / tetrahydrofuran; H2O / 20 °C 7: sodium hydroxide / H2O; tetrahydrofuran / 20 °C View Scheme

1: 67 percent / sodium dithionite / methanol 2: 76 percent / toluene / Heating 3: β-alanine; glacial acetic acid / hexane / Heating 4: 3-ethyl-5-(2-hydroxyethyl)-4-methylthiazolium bromide; triethylamine / ethanol / Heating 5: pivalic acid / tetrahydrofuran; toluene; heptane / Heating 6: 35 percent / hydrogen; acetic acid / palladium on carbon / ethyl acetate 7: HCl / tetrahydrofuran; H2O / 20 °C 8: sodium hydroxide / H2O; tetrahydrofuran / 20 °C View Scheme

1: β-alanine; glacial acetic acid / hexane / Heating 2: 3-ethyl-5-(2-hydroxyethyl)-4-methylthiazolium bromide; triethylamine / ethanol / Heating 3: pivalic acid / tetrahydrofuran; toluene; heptane / Heating 4: 35 percent / hydrogen; acetic acid / palladium on carbon / ethyl acetate 5: HCl / tetrahydrofuran; H2O / 20 °C 6: sodium hydroxide / H2O; tetrahydrofuran / 20 °C View Scheme

1: 3-ethyl-5-(2-hydroxyethyl)-4-methylthiazolium bromide; triethylamine / ethanol / Heating 2: pivalic acid / tetrahydrofuran; toluene; heptane / Heating 3: 35 percent / hydrogen; acetic acid / palladium on carbon / ethyl acetate 4: HCl / tetrahydrofuran; H2O / 20 °C 5: sodium hydroxide / H2O; tetrahydrofuran / 20 °C View Scheme

1: pivalic acid / tetrahydrofuran; toluene; heptane / Heating 2: 35 percent / hydrogen; acetic acid / palladium on carbon / ethyl acetate 3: HCl / tetrahydrofuran; H2O / 20 °C 4: sodium hydroxide / H2O; tetrahydrofuran / 20 °C View Scheme

chimera No.17A1V2 derived from CYP102A1 mutant in aq. phosphate buffer; dimethyl sulfoxide Enzymatic reaction; Catalytic behavior; Reagent/catalyst;

NADP+; dianionic G6P in aq. phosphate buffer; dimethyl sulfoxide T=37°C; pH=7.4; 1 h; Enzymatic reaction;

1: HCl / tetrahydrofuran; H2O / 20 °C 2: sodium hydroxide / H2O; tetrahydrofuran / 20 °C View Scheme