potassium phosphate buffer; NADH-H+; lactate dehydrogenase (Sigma Type II, from rabbit muscle); pyridoxal 5'-phosphate T=25°C; Michaelis constant Km and maximum velocity Vmax determination;

potassium phosphate buffer; NADH-H+; lactate dehydrogenase (Sigma Type II, from rabbit muscle); pyridoxal 5'-phosphate T=25°C; Michaelis constant Km and maximum velocity Vmax determination;