1: NaH / tetrahydrofuran / 0 - 25 °C
2: α-chymotrypsin / dimethylsulfoxide; H2O / 22 - 25 °C / potassium phosphate ( pH 7.0); with pig liver esterase the reaction rate is higher but the e.e. is lower
3: 1.) acyl azide formation, 2.) Curtius rearrangement
View Scheme