[(2R,3S,5R)-3-羟基-5-(5-甲基-2,4-二氧代-3,4-二氢-1(2H)-嘧啶基)四氢-2-呋喃基]甲基(3R,4S,5S,6R)-3,4,5-三羟基-6-(羟基甲基)四氢-2H-吡喃-2-基二氢二磷酸酯合成路线 (共 7 条)
反应条件
phosphate buffer; UDPG-PPase from Pyrococcus furiosus DSM 3638 T=90°C; pH=7.5; Enzyme kinetics;
参考文献
Mizanur, Rahman M.; Jaipuri, Firoz A.; Pohl, Nicola L.
Journal of the American Chemical Society, 2005 , vol. 127, # 3 p. 836 - 837
反应条件
Mg; thymidylyl-transferase T=37°C; 0.5 h;
参考文献
Jiang, Jiqing; Biggins, John B.; Thorson, Jon S.
Journal of the American Chemical Society, 2000 , vol. 122, # 28 p. 6803 - 6804
反应条件
1H-tetrazole in pyridine T=20°C; 72 h;
参考文献
Hong, Lin; Zhao, Zongbao; Melancon III, Charles E.; Zhang, Hua; Liu, Hung-Wen
Journal of the American Chemical Society, 2008 , vol. 130, # 14 p. 4954 - 4967
反应条件
acetylphosphoric acid; recombinant N-terminally hexahistidine-tagged Streptomyces ravidus TDP-D-glucose RavD synthase; recombinant N-terminally histidine-tagged acetate kinase; recombinant N-terminally histidine-tagged thymidine monophosphate kinase; adenosine 5'-triphosphoric acid; magnesium chloride in water T=37°C; pH=7.5; 1 h; phosphate bufferEnzymatic reaction;
参考文献
Kharel, Madan K.; Lian, Hui; Rohr, Juergen
Organic and Biomolecular Chemistry, 2011 , vol. 9, # 6 p. 1799 - 1808
反应条件
thermostable inorganic pyrophosphatase; Tris buffer; UDP-glucose pyrophosphorylase from E.coli in various solvent(s) T=37°C; pH=7.6; 0.0833333 h; Enzyme kinetics; Further Variations:Reagents;
参考文献
Ko, Kwang-Seuk; Zea, Corbin J.; Pohl, Nicola L.
Journal of the American Chemical Society, 2004 , vol. 126, # 41 p. 13188 - 13189
反应条件
NaOH buffer; bovine serum albumin; 2-[4-(2-hydroxyethyl)-1-piperazinyl] ethanesulfonic acid T=30°C; 6.66667 h; sucrose synthase;
参考文献
Zervosen, Astrid; Elling, Lothar; Kula, Maria-Regina
Angewandte Chemie, 1994 , vol. 106, # 5 p. 592 - 593
反应条件
recombinant sucrose synthase 1 (SuSy1) from potato; Tris buffer; alkaline phosphatase T=30°C; pH=8.0; 16 h; Enzymatic reaction;
参考文献
Roemer, Ulrike; Rupprath, Carsten; Elling, Lothar
Advanced Synthesis and Catalysis, 2003 , vol. 345, # 6-7 p. 684 - 686