5'-nucleotidase; tris(hydroxymethyl)methylamine hydrochloride; magnesium chloride in water T=37°C; 8 h; pH: 9.2;

1: 81 percent / 0.5percent-aq. NaOH, tris(hydroxymethyl)aminomethane-hydrochloric acid, NAD nucleosidase / H2O / 18 h / 37 °C / pH: 7.2 2: 96 percent / MgCl2, tris(hydroxymethyl)aminomethane-hydrochloric acid, phosphodiesterase / H2O / 12 h / 37 °C / pH: 9.0 3: 88 percent / MgCl2, tris(hydroxymethyl)aminomethane-hydrochloric acid, 5'-nucleotidase / H2O / 8 h / 37 °C / pH: 9.2 View Scheme

methanol; sodium methylate Ambient temperature;

1: 44.5 percent / p-toluenesulfonic acid / 0.17 h / 160 °C / 20 Torr 2: NaOMe, MeOH / Ambient temperature View Scheme

1: 44.5 percent / p-toluenesulfonic acid / 0.17 h / 160 °C / 20 Torr 2: NaOMe, MeOH / Ambient temperature View Scheme