potassium phosphate; 5'-amino-5'-deoxyuridine phosphorylase; UTP : 5-amino-5-deoxy-α-D-ribose-1-phosphate uridylyltransferase; magnesium chloride T=30°C; pH=7.5; 3 h; aq. phosphate bufferEnzymatic reaction;

1: 5'-NH2-Urd; UTP With potassium phosphate; 5'-amino-5'-deoxyuridine phosphorylase; UTP : 5-amino-5-deoxy-α-D-ribose-1-phosphate uridylyltransferase; magnesium chloride T=30°C; pH=7.5; 3 h; aq. phosphate bufferEnzymatic reaction; 2: 3N-(D-1-ribose)-uracil With 5-amino-5-deoxy-α-D-ribosyltransferase T=30°C; pH=7.5; 3 h; aq. phosphate bufferEnzymatic reaction;

potassium phosphate; 5'-amino-5'-deoxyuridine phosphorylase; UTP : 5-amino-5-deoxy-α-D-ribose-1-phosphate uridylyltransferase; magnesium chloride T=30°C; pH=7.5; 3 h; aq. phosphate bufferEnzymatic reaction;

1: 5'-NH2-Urd; UTP With potassium phosphate; 5'-amino-5'-deoxyuridine phosphorylase; UTP : 5-amino-5-deoxy-α-D-ribose-1-phosphate uridylyltransferase; magnesium chloride T=30°C; pH=7.5; 0.5 h; aq. phosphate bufferEnzymatic reaction; 2: 2-hydroxyethanethiol; phthalic carbaldehyde in ethanol pH=9.5;

potassium phosphate; 5'-amino-5'-deoxyuridine phosphorylase; UTP : 5-amino-5-deoxy-α-D-ribose-1-phosphate uridylyltransferase; magnesium chloride T=30°C; pH=7.5; 3 h; aq. phosphate bufferEnzymatic reaction;

potassium phosphate; 5'-amino-5'-deoxyuridine phosphorylase; UTP : 5-amino-5-deoxy-α-D-ribose-1-phosphate uridylyltransferase; magnesium chloride T=30°C; pH=7.5; 3 h; aq. phosphate bufferEnzymatic reaction;

potassium phosphate; 5'-amino-5'-deoxyuridine phosphorylase; UTP : 5-amino-5-deoxy-α-D-ribose-1-phosphate uridylyltransferase; magnesium chloride T=30°C; pH=7.5; 3 h; aq. phosphate bufferEnzymatic reaction;

potassium phosphate; 5'-amino-5'-deoxyuridine phosphorylase; UTP : 5-amino-5-deoxy-α-D-ribose-1-phosphate uridylyltransferase; magnesium chloride T=30°C; pH=7.5; 3 h; aq. phosphate bufferEnzymatic reaction;

1: 5'-NH2-Urd; UTP With potassium phosphate; 5'-amino-5'-deoxyuridine phosphorylase; UTP : 5-amino-5-deoxy-α-D-ribose-1-phosphate uridylyltransferase; magnesium chloride T=30°C; pH=7.5; 3 h; aq. phosphate bufferEnzymatic reaction; 2: 3N-(D-1-ribose)-uracil With 5-amino-5-deoxy-α-D-ribosyltransferase T=30°C; pH=7.5; 3 h; aq. phosphate bufferEnzymatic reaction;

potassium phosphate; 5'-amino-5'-deoxyuridine phosphorylase in Tris HCl buffer T=30°C; pH=9; 0.5 h; Enzymatic reaction; KineticsMechanism; ConcentrationpH-valueReagent/catalystTime;