4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide in dimethyl sulfoxide T=20°C; 8 h;

1: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 2: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 3: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 4: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 5: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 6: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 7: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 8: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 9: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 10: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 11: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 12: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 13: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C View Scheme

pyridine in benzene

1: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 2: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 4: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 5: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 6: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C View Scheme

1: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 2: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 4: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 5: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 6: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 7: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 8: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 9: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 10: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C View Scheme

1: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 2: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 4: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 6: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 7: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 8: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 9: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 10: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 11: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 12: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C View Scheme

1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 2: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 4: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 5: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 6: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 7: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 8: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C View Scheme

1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 2: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 4: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 6: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 7: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 8: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 9: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 10: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C View Scheme

in acetonitrile T=55°C; Substitution; Kinetics; Further Variations:Reaction partners;

1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 2: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 4: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 6: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 7: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 8: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 9: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 10: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 11: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 12: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 13: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 14: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C 15: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction 16: 4-(N,N-dimethlyamino)pyridine; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C View Scheme