1: 44 percent / 1N NaOH / Pseudomonas mendocina lipase / aq. phosphate buffer / 72 h / 4 °C / pH 7.0 / Enzymatic reaction 2: 93 percent / TFA / CH2Cl2; H2O / 2 h / 20 °C / Acid hydrolysis 3: 99 percent / LiOH / H2O / pH 10 4: 73 percent / pyridoxal phosphate; cysteine sulfinic acid / Glutamic Oxalacetic Transaminase (GOT) / H2O / 24 h / 20 °C / pH 7 / Enzymatic reaction View Scheme

1: 1N NaOH / Pseudomonas mendocina lipase / aq. phosphate buffer / 72 h / 4 °C / pH 7.0 / Enzymatic reaction 2: 100 percent / SOCl2 / Heating 3: 95 percent / TFA / CH2Cl2; H2O / 2 h / 20 °C / Acid hydrolysis 4: 98 percent / LiOH / H2O / pH 10 5: 78 percent / pyridoxal phosphate; cysteine sulfinic acid / Glutamic Oxalacetic Transaminase (GOT) / H2O / 24 h / 20 °C / pH 7 / Enzymatic reaction View Scheme